B cell
Autoimmunity Assays
- Antibody production (Total IgG)
- Memory B cell Antibody Isotypes
- Naïve B cells Isotype switching
- B cell proliferation/activation/cytokine secretion
- Antigen presentation
Tonsillar re-aggregate model
Tonsils are a secondary lymphoid tissue rich in B cell subsets and the specialized, anatomically restricted T follicular helper (Tfh) subset of CD4 T cells. Tonsils provide an accessible source of cells for those interested in B cell immunology, Tfh biology and vaccine development. The tonsillar cell re-aggregate model provides functional insight into human germinal center biology, allowing measurement of key features including naive B cell differentiation, plasma cell/plasmablast differentiation, and antibody production.
Figure A. Schematic representation of tonsil re-aggregate model. Cells are seeded at high density in 96 well plates; unstimulated (US) or stimulated with quadrivalent inactivated influenza vaccine (IIV) in the presence of vehicle or reference inhibitors such as Rapamycin. B cell readouts include B cell phenotyping, B cell proliferation and hemagglutinin (HA)-specific IgG by ELISPOT or ELISA.
Tonsil tissue and tonsil re-aggregates in high density cultures on Day 5 and following IIV stimulation
Figure B. Flow plots representing B cell subpopulations. C. Bar graphs showing frequencies of each B cell subpopulation.
Figure D. Flow plots representing total B cell proliferation. E. Bar graphs showing frequencies of divided B cells. Gating strategy: CD45+ > Viable > FSC vs SSC > Singlets > CD4-CD8- > CD19hi/lo
Drug Discovery Tool
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